Labbioreactors

Labbioreactors Plant Tissue Culture Bioreactors and Services plant tissue culture services

23/05/2026

Subculture...




Inside the glass vessel, a quiet revolution unfolds — a living broth of plant cells, suspended in nutrient-rich liquid, ...
09/05/2026

Inside the glass vessel, a quiet revolution unfolds — a living broth of plant cells, suspended in nutrient-rich liquid, swirls gently in the rhythm of the bioreactor. What began as a humble callus, an undifferentiated mass of potential clinging to solid medium, has now transformed into a thriving cell suspension, millions of individual cells and small aggregates drifting and multiplying in sterile isolation.

This is the heart of precision propagation. No soil, no sunlight — just meticulously controlled conditions, where every milliliter carries the blueprint of an entire plant. The gentle agitation keeps the culture oxygenated, preventing the cells from settling, from giving up their individuality to form yet another callus.

From these suspended pioneers, scientists can unlock secondary metabolites, propagate elite plant varieties, or engineer resilience at the cellular level. The progression from static callus to dynamic suspension marks a critical milestone — proof that the cells have not only survived but fully adapted to their liquid world, committed to growth, ready to become anything.





🥔 Tuberous root (storage root) 🥔Talinum paniculatum (often called “ginseng Jawa”) has a very characteristic root formati...
03/05/2026

🥔 Tuberous root (storage root) 🥔

Talinum paniculatum (often called “ginseng Jawa”) has a very characteristic root formation pattern that’s strongly influenced by physiology, environment, and propagation method.

The tuberous roots of T. paniculatum are very similar to the physical appearance of ginseng roots, hence the name Javanese ginseng.

In vitro culture, tuberous roots are very likely to be induced. Tuberous roots that develop in vitro are very similar to those that develop ex vitro, but in a miniature version (hence the name, micro tuberous root).

Experimental results show that root induction in T. paniculatum explants does not always result in tuberous root development.

On slide 2, the explant growth environment (culture medium) does not support tuberous root development. Therefore, only adventitious roots with thin (thin) characteristics grow (pen marks).

In contrast to the results on slide 3, potential or initial tuberous roots develop on adventitious roots induced by the culture medium (pencil marks). Tuberous root development in this medium is characterized by thick and bulging adventitious roots. These adventitious roots will grow larger over the culture period (2-3 months).

The results of tuberous root development can be seen on slide 4.

It seems like this experiment is interesting to continue, maybe we can analyze secondary metabolites in micro tuberous roots or we can find out whether there are genetic factors that play a role in the formation of tuberous roots.

Share your thoght below .... 😊





Have you seen or got callus firm like the pictures ❓This is an anucleate callus (the cells have no nucleus)The callus lo...
12/01/2026

Have you seen or got callus firm like the pictures ❓
This is an anucleate callus (the cells have no nucleus)
The callus looks like cotton fiber
Very fragile, the color is pure white different with common callus.

I have discussed with who is expert in plant plant physiology and plant tissue culture, and here is the conclusion :

The callus like cotton fiber is generated from normal /common calluses, but they produce more fiber form. The fiber form requires a lot of cellulose, and other resources for growth, it means resulting in inhibition of main growth.

Several reasonable assumptions why the fiber callus appears / growth during the culture is :

▶️ Maybe the plants have auxin/cytokinin overproduction as some bacterial gene in the genome
▶️ To high concentration of local (exogen) hormone and or endogenous hormone which disappeared once canalized for normal callus

So, how to counter this happen ❓

✔️ Considering growth parameters alteration:
can be medium with reduction hormone precursors, light, temperature, maybe more potassium, but need to review all details.
✔️Avoid or consider use plants endogenous hormone and a lot of hormone precursors.
Example : remove or reduce to minimum for B1 (and other vitamines), reduce Zn to 0,5 mg/l or so

Drop your experience in the comment below.......









Mechanistically, variegation can be further categorized into four distinct anatomical or biochemical types (Hara, 1957; ...
09/01/2026

Mechanistically, variegation can be further categorized into four distinct anatomical or biochemical types (Hara, 1957; Stewart and Dermen, 1979; Zonneveld, 2005):
• Type I: Complete absence of chlorophyll in certain sectors due to defective chloroplast biogenesis, resulting in white or pale areas.
• Type II: Internal air spaces below the cuticle scatter light, producing silvery or pale visual effects.
• Type III: Structural irregularities in epidermal cells modify light reflection, creating visible variegation.
• Type IV: Localized accumulation of non-photosynthetic pigments, such as anthocyanins, leads to red, purple, or blue coloration.

These forms are often grouped into two major classes:
• Pigment-based variegation, arising from localized disruptions in chlorophyll, carotenoid, or anthocyanin biosynthesis.
• Structural variegation, caused by air spaces, irregular mesophyll layers, or epidermal abnormalities (Sheue et al., 2012).

So, what typically variegated plant form do you have ?









🌿Glimps of plant callus in plant tissue culture🌿Plant callus is basically a clump of undifferentiated plant cells that s...
10/12/2025

🌿Glimps of plant callus in plant tissue culture🌿

Plant callus is basically a clump of undifferentiated plant cells that shows up when tissues are stressed, wounded, or nudged by plant hormones. It’s a botanical “reset mode,” letting cells drop their usual identity. Early plant physiologists caught on to this phenomenon almost a century ago—White (1934) famously noted that “tissue placed on a suitable medium can continue to grow independently of the parent plant” (White, The Cultivation of Animal and Plant Cells, 1934), which laid the groundwork for how we understand callus today.

The term “callus” in modern plant biotechnology really took off after Skoog & Miller (1957) demonstrated the hormonal switchboard behind it. Their classic line—“The growth and organ formation in plant tissue cultures depends upon a proper balance of auxin and cytokinin” (Skoog & Miller, Symposia of the Society for Experimental Biology, 1957)—is still the go-to explanation. Basically, tweak the hormone ratio and you can hold cells in callus form or push them into roots, shoots, or whole plants.

Functionally, callus is the plant world’s equivalent of a blank slate. It lets researchers regenerate entire organisms, fix genetic lines, and test biochemical pathways. Murashige & Skoog (1962) expanded this practical side by creating a medium that, in their words, “supports sustained, vigorous callus proliferation in to***co cultures” (Murashige & Skoog, Physiologia Plantarum, 1962). With a reliable growth recipe, callus became a routine laboratory tool.

Today, applications range from genetic engineering and clonal propagation to metabolite production. Advanced tissue culture manuals—like George, Hall & De Klerk—underline this versatility, stating that “callus cultures provide a manipulable platform for dedifferentiation, redifferentiation, and recombinant approaches” (George et al., Plant Propagation by Tissue Culture, 2008). Whether you're editing genomes or rescuing rare species, callus is the flexible starting point that keeps plant biotech moving forward.








New approach ......🌱📝 melakukan penelitian ini sebagai bentuk dukungan dan kontribusi terhadap pengkayaan data penelit...
29/11/2025

New approach ......🌱
📝 melakukan penelitian ini sebagai bentuk dukungan dan kontribusi terhadap pengkayaan data penelitian tentang kultur jaringan sebagai upaya konservasi ex situ.

👨‍🔬 Penelitian ini menggunakan ZPT seperti 2,4-Dichlorophenoxy acetic acid (2,4-D), 6-Benzyloaminopurine (BAP), Thidiazuron (TDZ), dan N(2-chloro-4-pyridyl)-N’-phenylurea (CPPU) untuk menguji respons pertumbuhan eksplan A. titanum. Penelitian bertujuan menentukan ZPT terbaik dalam menginduksi kalus pada eksplan petiolus A. titanum, dengan harapan dapat menjadi dasar bagi pengembangan protokol perbanyakan bibit skala besar menggunakan bioreaktor.











🌱 Ginger cultures was done ⚗ Device : Balloon-type bubble bioreactor ( )🍃 Explant source : Callus🧪 Treatment : Feed & ...
17/10/2025

🌱 Ginger cultures was done

⚗ Device : Balloon-type bubble bioreactor ( )
🍃 Explant source : Callus
🧪 Treatment : Feed & Batch cultures
💡 Photoperiod : 16/8

👨‍🔬 work by :

Device info : [email protected]







The bioactive compounds are mainly extracted from plant biomass restricted by limited suitable agricultural land and var...
09/10/2025

The bioactive compounds are mainly extracted
from plant biomass restricted by limited suitable agricultural land and various environmental stresses. A possible alternative is the production of plant biomass and metabolites through in vitro method. This method can be scaled-up using a bioreactor culture system to increase the production of target secondary metabolites. Since the highest bioactive compound accumulation was detected in the G. procumbens roots, it is desirable to use its adventitious roots as
an initial culture to produce secondary metabolites. The research has been done with employee 3L ( ) on various treatments (slide 1-4).

Based on antioxidant and anti-cancer tests, administration of adventitious roots G. procumbens (ARGp) with antioxidant activity
repaired hematologic damage, reduced the MDA level, and
improved GPx-4 expression. These findings indicated prevention of stress oxidation. ARGp further exhibited a moderate anti-cancer effect towards the Huh7it cell line with an IC50 = 44.65 mg/L (slide 5).

04/10/2025

🔑 Key Agitation-Mixing in simple Bioreactor...

Plant suspension cells are more shear‐sensitive than many microbes. Bubble‐bursting at the surface, high velocities of bubble generation, sharp turbulence near sparger orifices can cause damage. Thus, the sparger design must balance sufficient oxygen delivery with gentle hydrodynamics.

In many bubble bioreactor contexts, mixing is achieved by agitation driven by bubble motion, i.e. pneumatic agitation, rather than mechanical impellers.

Agitation here is pneumatic, meaning the agitation force is from bubble rise and gas injection rather than from mechanical motors. It includes the forces imparted by bubbles on the surrounding liquid, which moves upward (in riser), downward (in downcomer, or as liquid flows back), and the turbulence due to wakes of rising bubbles.

Mixing is the result of agitation plus diffusion, convection. It includes:
- Bulk mixing: large‐scale circulation induced by rising bubbles.
- Local mixing: around bubbles, wake regions, bubble‐liquid interfaces.
- Micro‐mixing: small eddies, diffusion across thin laminar films around bubbles or cell aggregates.

📈 Benefits:
- Low mechanical shear compared to impellers. Good for plant suspension cultures, which often are shear‐sensitive. The absence of rotating parts reduces risk of damaging cells.
- Simplicity of design & lower maintenance. Bubble‐driven systems (bubble columns, airlifts) have fewer moving parts, simpler sterilization, less mechanical failure.
- Better oxygen mass transfer if designed well – using fine spargers, membrane or sintered materials, you can get relatively high k-La without high energy input.
- Gentler mixing helps preserve viability, morphology, metabolite production, especially in secondary metabolite production where physiology is sensitive to stress.

Device : Balloon-type Bubbles Bioreactor ( ). MOD. by Labbioreactors
video by :
Info product : [email protected] & 📩






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