03/21/2022
Well, if you know you know, but if you don't know ask because I love to share.
I recently purchased two of the main pieces of equipment used to perform plant tissue culture at home with reliable success.
Plant tissue culture is normally carried out in a commercial or university laboratory. The cost of equipment and supplies + skilled labor and training is beyond most household budgets.
Though plant tissue culture can be performed with improvised equipment fashioned from available household items, I opted to purchase used standard equipment at a fraction of the new cost.
The first picture is a laminar flow hood equipped with a HEPA air filter and a UV light. This piece of equipment provides a flow of clean air free of dust, spores, and bacteria. The aseptic conditions inside of the cabinet are required to prevent contamination during the handling and transferring plant materials for tissue culture.
In the second picture is a pressure sterilizer also known as an autoclave. It is used to sterilize growing media, tools, and all containers used in tissue culture.
When I started to seriously consider performing tissue culture at home I watched numerous videos and read many scholarly articles as anyone else would. I also purchased a book: Plant Propagation: Principles and Practices. I chose this book because it is the book used in the plant propagation course at UF. It has 2 full chapters dedicated to plant tissue culture alone. This book contains hundreds of methods of plant propagation that I've never heard of, or ever could have imagined.
The first plant that I am going to propagate by tissue culture is banana. That is the plant that sparked my interest in and desire to do tissue culture. I will use the Nam Wah banana that I have growing in my garden. It was purchased from back in spring/summer of 2020. I recently learned it too was produced by way of tissue culture which I found to be super cool. A banana plant from tc back into tc.♾️
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